A Comprehensive Introduction to Depth Glass Fiber Filter

Issuing time:2021-05-26 02:16:58

A Comprehensive Introduction to Depth Glass Fiber Filter

A depth glass fiber filter is used when you need high-flow submicron or micron filtration. These filters are ideal for both liquid and air filtration. You can choose from binder filters or binder-free filters, depending on your application. A binder-free filter for purity is suitable for gravimetric and analytical determination. On the other hand, a binder filter provides strength in the long run and is appropriate for wet or high-pressure applications.

fiber filter

In this guide, we will help you develop a better understanding of the glass debt fiber filters. We will discuss their types, uses, and other relevant information that you need to know.

Types of Depth Glass Fiber Filter

There are two types of depth glass fiber filters available to choose from. The available types are A/E glass fiber filters and A/B glass fiber filters. A/E glass fiber filters are suitable to take air pollution gravimetric analysis and to test suspended/dissolved wastewater solids. They are also appropriate for wet strength, high-flow rates, and solids (dirt) holding capacity.

On the other hand, an A/B glass fiber filter is the most appropriate choice for thicker glass, high dirt holding capacity. These filters are manufactures with top-quality borosilicate glass microfibers. Both types of depth glass filters have their unique benefits and are suitable for different applications.

Here are the highlights of these depth glass fiber filters:

  • High-temp tolerance (Autoclavable)
  • 0µm liquid nominal size of the pore
  • High wet strength and particle retention
  • Binder-free glass fiber borosilicate to remove interfaces
  • Suitable for gravimetric study

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Now, let’s take a quick look at the ratings of the pore size of these filters.

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Rating of Pore Size

The rating of a pore size depends on the rating of liquid filtration. A filter retains the smallest air particles, like 0.8µm liquid-rated filter retains >50% of 0.8µm smallest particles. Usually, an approximate or nominal rating is useful for depth filters. Unlike a membrane filter, a depth glass filter doesn’t include an evenly defined structure of pore. However, it contains sintered particles of fibers that interweave for creating openings’ torturous path that helps the air pass through. Meanwhile, it traps particles in the filter’s depth.

Due to this structure, a depth fiber filter is examined for the size of the pore. It is tested by letting the liquid that contains certain size particles through the depth filter. If there is a success in filter trapping a reasonable amount of particles, the size of the particle becomes ‘liquid nominal size of pore rating for depth filters. Typically, the size ratings of a nominal pore are defined as ‘Filter removes> xx% of the particle ≥ µm’.

Glass Fiber Filters and Nucleic Acid Extraction

There are different approaches to the extraction of nucleic extraction. Did you know that a glass fiber filter offers a simple solution for the extraction of nucleic acid? It is a perfect alternative to magnetic beads and silica powder for the isolation of nucleic acids. There is a great need for high-quality and accessible methods to extract nucleic acid for molecular diagnostics engineers, kit manufacturers, and researchers during this pandemic time.

Solid-phase extraction is employed by commercial kits using materials, like borosilicate glass, silica, and other surface chemistries. Silica is popular due to its capabilities to bind nucleic acids, such as DNA with chaorropic salts. It is a straightforward method but needs careful handling.

The extraction of nucleic acid using magnetic beads has risen in popularity because of their effectiveness and flexibility in biomolecules isolation. Magnetic beads are ideal for the size selection and isolation of nucleic acid for the NGS (next-generation sequencing). They are also ideal for custom conjugations and lab-based immunoassays. It is easy to automate magnetic beads than column-based approaches. It makes greater throughput extraction.

Alternative to Magnetic Beads and Silica Powder

Borosilicate GF (glass fiber filters) also provide extraction of nucleic acid as a perfect alternative to magnetic beads and silica powder. The extraction using a glass fiber filter ensures high quality and is also straightforward. Glass fiber materials are highly versatile materials with several applications as a depth filters.

Silica is a major component of a glass fiber filter. Therefore, these filters are appropriate for the extraction of nucleic acid with the help of binding principles. Glass fiber proves advantageous because it is a highly adaptable, easy-to-handle, and robust material. They are cost-effective, flexible, and an easy tool to extract nucleic acid for the new businesses developing nucleic acid and molecular diagnostics kits.

Here are the different reasons why you should use a glass fiber filter to extract nucleic acid:

They are Cost-Effective

It is a simple method to extract nucleic acid and doesn’t need specialized laboratory consumables or equipment. Usually, researchers know how to use and handle these filters in diagnostic and research laboratories for an array of applications. You can easily source GF filters and they are cost-effective. Easy sourcing and cost-efficiency are the two essential considerations for start-ups and small laboratories. Therefore, you can find these filters easily and inexpensively in reel and sheet material. They are also available as discs.

Convenient Formats

Glass filters are available in different materials, such as reel and sheet formats. Due to this, developers get several options for optimization and assay design. Researchers can ‘punch-and-go’ discs with unique diameters to fix equipment and perform process optimization tests. They can heap glass fiber layers or various glass fiber grades in various combinations. It helps in achieving optimum binding of nucleic acid, yield, and loading capacity for the application and sample type.

The manufacturer can also get benefit from reel and sheet formats for punching glass filters to specific specs to integrate into an assay component. The availability and cost-effectiveness of a glass fiber filter in various formats and grades make it a perfect choice. Therefore, it is highly suitable to test workflow and consumables, manufacture optimized products and protocol.

fiber filter

Conclusion

A top-quality glass fiber filter delivers consistent and efficient extraction of nucleic acid. Depth glass fiber filters are most suitable for applications where submicron/micron filtration and a high-flow rate are a requirement. You can select from a binder or a binder-free filter, depending on your project requirement. They are cost-effective and available in several convenient formats.

For more information about GF filters, you can contact us, anytime.

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